转基因番木瓜的抗病性及分子鉴定.pdfVIP

H EREDI TA S ( Beij ing) 25( 2) : 181~ 184, 2003 1 1 1 1 2 叶长明, 魏祥东 , 陈东红, 蓝崇钰, 朱利民 ( 1. , 510275; 2. , ) : T (PRV) ( RP) , 1 , PRV , RP RNA , PCR CaMV35S N P TII : ; ; : Q812 :A :0253- 9772( 2003) 02- 0181- 04 Analyses of Virus Resistance And Transgenes for Transgenic Papaya 1 1 1 1 2 YE Cha g-Mi g , WEI Xia g-Do g , CHEN Do g-Ho g , LAN Cho g-Yu , ZHU L-i M i ( 1. The Key L aboratory of Gene Engineer ing of The M inistry of Edu at ion, The State K ey L abor atory of Biologi al Contr ol , Zhongshan University , G uangzhou 510275, China ; 2. Dep artment of Biology , The Chinese Univ ersity of H ong K ang ) Abstract: Virus resista ce i field a d molecular biological characterizatio s of the tra sge es w ere a alyzed for tw o li es of T ge eratio of tra sge ic papaya w ith the replicase muta t ge e from papaya ri gspot virus ( PRV) . The tra sge ic pla- 1 ts showed highly resista t or immu e agai st PRV . Results i dicated t hat t he tra sge es i herited to a d expressed at RNA level i the proge ies. Key words:tra sge ic papaya; virus resista ce; tra sge es detectio , , 2000 1 T 1 1 2 , , 2000 ( papaya ri gspot virus, PRV) 3 PRV, 15d , , PRV ( CP) , 2 , 200 P, , 1997 FDA 5 PRV, [ 1, 2] PRV ( RP) ( RP ) 1. 2 RP [ 3, 4] ,