主动载药法制备双氯芬酸钠脂质体及其体外释放.pdf

学 报 2007 ,38 ( ) Journal of China Pharmaceutical University 　 4 :323 - 326 323 主动载药法制备双氯芬酸钠脂质体及其体外释放 王 　玮 ,周建平 ,任逢晓 ( 中国药科大学药剂学教研室 ,南京 210009) 摘 　要 　目的:采用主动载药法制备双氯芬酸钠脂质体 ,考察包封率的影响因素 ,并对其体外释放进行评价 。方法 :利 用醋酸钙梯度法制备双氯酚酸钠脂质体 ,考察不同因素对包封率的影响 ,并对药物体外释放行为进行研究 。结果 :脂质体 包封率随温度和醋酸钙浓度的升高而增加 、随药脂比的增大而降低 。与混悬剂相比 ,其体外释放较完全 。结论 :醋酸钙梯 度法适于制备双氯芬酸钠脂质体 ,后者的体外释放具有明显缓释特征 。 关键词 　双氯芬酸钠 ;脂质体 ;醋酸钙梯度法 ;主动载药法 ;体外释放 ( ) 中图分类号 　R944 　　文献标识码 　A 　　文章编号 　1000 - 5048 2007 04 - 0323 - 04 Prep aration of diclofenac so dium lipo so me s by active lo a ding metho d and it s rele a se in vitro WAN G Wei ,ZHOU Jianping ,REN Fengxiao Dep artment of Pharmaceutics , China Pharmaceutical University , N anj ing 210009 , China Ab stract 　Aim :To prepare diclofenac sodium (DS) liposomes by active loading method ,to study the influence of dif ferent factors on the entrapment efficiencies and to investigate the drug release behavior in vitro . Metho d s :DS lipo some was prepared by calcium acetate gradient method . The influence of various factors on the entrapment efficiencies was compared and the release behavior was studied in vitro . Re sult s :The enhanced efficiency efficiency was shown by using the high temperature ,the increased calcium acetate concentration and the decreased drugtolipid ratio . It was al so found that the release of the liposomes was higher than that of the suspension . Conclu sion :Calcium acetate gradient method is suitable for preparing DS liposomes which exhibits sustainedrelease profile in vitro . Key word s 　diclofenac sodium ;liposomes ;calcium acetate