药用植物雷公藤离体繁殖的研究.pdfVIP

For. Stud. China, 2009, 11(3): 174–178 RESEARCH ARTICLE DOI 10.1007/s11632-009-0024-3 In vitro propagation of a medicinal plant: Tripterygium wilfordii Hook f. CHEN Bi-hua 1,2* 1Forestry Biotechnology Research and Development Center, Fujian Academy of Forestry Sciences, Fuzhou 350012, P. R. China 2Key Laboratory of Timber Forest Breeding and Cultivation for Mountainous Areas in Southern China, State Forestry Administration, Fuzhou 350012, P. R. China Abstract In this study a reliable protocol was developed for the establishment of commercial in vitro cultures of Tripterygium wil- fordii Hook f.. Juvenile shoots from one-year-old elite plants were used as the source of explants. New axillary shoots were obtained after 30 days of culture on a MS medium supplemented with BAP (2.0 mg·L–1) and NAA (0.1 mg·L–1). The optimal multiplication medium was a modified MS medium supplemented with BAP (1.0 mg·L–1) and NAA (0.1 mg·L–1). This yielded a multiplication rate of 2.4 for each subculture. Slightly more than 92% of shoots rooted when cultured on a modified MS medium containing IBA (0.2 mg·L–1) and activated charcoal (0.5 mg·L–1). Activated charcoal promoted both a strong and a high rooting rate during the rooting phase. Plantlets were transferred to pots for a short acclimatization stage in a greenhouse where 95% of the plantlets survived. This highly reproducible procedure can be adopted for large-scale propagation of T. wilfordii. Key words in vitro propagation, Tripterygium wilfordii Hook f., multiplication, rooting, activated charcoal 1 Introduction opment of plant breeding programs and the fast pro- duction of propagation material in bulk quantities Tripterygium wilfordii Hook f. is