基因枪转化的bar基因表达盒在水稻中遗传行为复杂_英文_.pdfVIP

Journal of Genetics and Genomics (Formerly Acta Genetica Sinica) September 2007, 34(9): 824835 Research Article Hereditary Behavior of bar Gene Cassette is Complex in Rice Mediated by Particle Bombardment 1 2, 3 2 Yan Zhao , Qian Qian , Huizhong Wang , Danian Huang 1. College of Food, Biological and Environmental Engineering, Zhejiang Gongshang University, Hangzhou 310035, China; 2. State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China; 3. Life Science Department of Hangzhou Teacher’s College, Hangzhou 310036, China Abstract: Particle bombardment transformation using minimal gene cassette (containing the promoter, open reading frame and terminator) is the novel trend in plant genetic transformation, and its use helps to alleviate the undesirable effects of plasmid vector backbone sequences on transgenic plants. In the present article, studies related to the hereditary behavior of bar gene cassette in T1 to T3 generations of the transgenic rice (Oryza sativa L.) lines transformed by particle bombardment have been discussed. The se- lectable marker bar gene cassette that integrated with the rice genome had multiple copies and showed complex segregation behav- iors including the presence of ‘false homozygotes’, with abnormal segregation ratios ranging from 35:1 to 144:1 (Basta-resistant: sensitive plants) in their progenies. In five out of ten original transgenic lines, bar gene can be stably transmitted as a dominant gene to self-pollinated T2 progeny. The homozygotes were obtained in three transgenic lines in T1 generation regardless of the multi- ple-copy integration patterns of bar gene. Southern blotting analysis showed that multiple copies of bar gene casset