重金属污染对大蒜根尖细胞的诱变效应研究.pdfVIP

, Journal of AnhuiA gri. Sc.i 2010, 38( 33) : 18638- 18640 王强 况玲玲 丛娟, 甄卓, 彭悦 (, 130012) [目的]探究 PbH g及其复合污染对植物细胞诱变效应的机制[方法]利用植物细胞 畸变技术, 研究不同浓度的 PbHg及其 复合污染对大蒜根尖细胞分裂指数微 率及总畸变率的影响[结果] PbHg及其复合污染均能抑制大蒜根尖细胞的有丝分裂并诱发 较高频率的 畸变, 其毒害作用均随污染浓度的升高而增强在抑制细胞分裂方面, H g作用强于Pb; 在细胞致畸方面, Pb作用强于Hg 当 Pb浓度 0. 8 mg/L时, 致畸影响极显著(P 0. 01); 两者复合时, 对细胞的毒害作用不是简单累加, 而是既协同又部分拮抗[结论] 该研究为重金属环境污染的质量评估提供技术参考 大蒜; Pb; H g; 分裂指数; 诱变 S 633. 4 A 0517- 6611( 2010) 33- 18638- 03 M utagen ic Effects of H eaw gM etau R oot T ip Cell of Gar lic CONG Juan et al ( Coll g of Ch m istry and L if Sci nc of Changchun U niv rsity of T cnology, Changchun, Jilin 130012) A bstract [Ob j ctiv ] To inv stigat th m chanism ofmutag nic ff cts of PbH g and th ir compound pollution on plant c lls. [M thod] Th nucl ar ab rration t chnology in plant c lls was utiliz d to study th m itotic ind x, m icronucl us rat , and th total ab rration rat in diff r nt con- c ntrations of PbHg and th ir compound pollution on th root tip c llof garlic. [ R sult] Th r sult show d thatPbHg and th ir compound pollu- tion could inhibitm itosis of root tip c ll of garlic, and induc high fr qu ncy nucl ar ab rration. In th asp ct of inhibiting c ll div ision, th ff ct ofHg is gr at r than that of Pb, how v r, in th asp ct of c ll ab rration, th ff ct ofPb ismor significant. W h n th conc ntration of Pb w r mor than 0. 8 mg/L, it has significant ff ct on c ll ab rration, (P 0. 01). Th compound pollution show d that th po isoning ff ctwas not a smi pl sum of Pb andHg. On th on hand, th r was coordination b wt n th m, on th oth r hand, som antagonistic ff ctsw r also x ist d. [ Conclusion] Th r s arch prov id s t chnolog ical r f r nc s for quality valuation of h avy m tal pollution in th nv ironm n.t K ey w ords G arlic; Pb; Hg; M itotic ind x; Mutation , 0. 50. 81. 05. 010. 0 15. 0 mg /L); Pb + H g ,