不同时间经皮穴位电刺激介入镇痛作用及其对脊髓细胞外信号转导激酶1%2f2活化的调控.pdfVIP

不同时间经皮穴位电刺激介入镇痛作用及对脊髓 细胞外信号转导激酶1/2活化的调控 房军帆 方剑乔梁 宜杜俊英 浙江中医药大学第三临床医学院，杭州310053 摘要：目的：观察不同时间经皮穴位电刺激(TEAS)介入的镇痛效应及其对脊髓背角细胞外信号转导激 酶1/2(ERK1/2)活化的干预作用。方法：大鼠分为空白对照组、模型对照组、TEAS治疗组和提前TEAS治疗 组。采用足底注射完全弗氏佐剂建立大鼠炎症痛模型。提前TEAS治疗组和TEAS治疗组分别于造模前7d起和造 模后5.5h取双侧足三里、昆仑穴进行TEAS治疗。分别观察造模前7d和造模后5h、6h的足缩腿阈(PWTs)及患 侧脊髓背角p-ERK1/2蛋白表达。结果：与空白对照组相比，模型对照组大鼠PWTs显著性降低，p-ERK1/2表达 增加(P＜0.01)。造模后6h，与模型对照组相比，TEAS治疗显著性提升了大鼠PWTs，并减少了p-ERK1/2表达 (P＜0.01)。造模后5h，提前TEAS治疗组PWTs显著高于同期TEAS治疗组和模型对照组(P＜0.01)，但p-ERK1/2 表达水平无统计学差异。造模后6h，提前TEAS治疗组与TEAS治疗组相比在各方面均无差异。结论：TEAS镇痛效 应与其抑制脊髓背角ERK1/2的磷酸化密切相关。提前介入TEAS可以在一定程度上缓解炎症痛的产生，但其机制 尚不清楚。 关键词：经皮穴位电刺激；炎症痛；镇痛；细胞外信号转导激酶1/2磷酸化 EffectsofdifferenttimepointinterventionofTEASonphosphorylationofspinalERK1/2 andinflammatorypain FANGJun-fanFANGJian-qiaoLIANGYiDUJun-ying ThirdClinicalMedicalCollege,ZhejiangChineseMedicalUniversity,Hangzhou310053,China Abstract:Objective:Toobservetheanalgesiceffectsoftranscutaneouselectricalacupointstimulation(TEAS),which administratedatdifferenttime,anditsinterventionontheactivationofERK1/2inspinalcorddorsalhorn.Methods:Allratswere randomlydividedintonormalgroup(ngroup),modelgroup(CFAgroup),TEASgroupandpre-TEASgroup.Inflammatorypain wasinducedbyinjectingsubcutaneouslywith100μLCompleteFreundsAdjuvant(CFA)intorighthindpawofratsinCFA, TEASandpre-TEASgroup.Pre-TEASandTEASgroupswererespectivelyappliedtobilateral ‘Zusanli(ST36)and ‘Kunlun (BL60)at7dbeforeCFA-treatedand5.5hafterCFAinjection.Thepawwithdrawalthresholds(PWTs)weremeasuredbyusing DynamicPlantarAesthesiometer37450on7dbeforeCFA-treated(asBASE)andat5h,6hafterCFAinjection.Theexpression levelofERK1/2inspinalcorddorsalhorn(SCDH)wasdetectedbyWesternBlotat5hand6hafterCFAinjection.Results: Comparedwiththatofngroup,PWTsofratsinCFAgroupweredecreasedmarkedlyduringthewholeexperiment,andthe expressionlevelofp-ERK1/2ofips