东北虎肠道降胆固醇双歧杆菌的分离鉴定.pdfVIP

※生物工程 食品科学 2009, Vol. 30, No. 05 205 尹军霞，沈国娟，邹 波，陈海清，李 洋，丁珍珍 (绍兴文理学院生物系，浙江绍兴 312000) ：为开发能安全高效降血脂和预防心血管疾病的保健食品，用双歧杆菌的选择性培养基(TPY) ，从东北虎 肠道(粪便) 分离、筛选出双歧杆菌，选取体外降胆固醇能力最强的菌株培养物灌胃高脂模型大鼠，研究其对大鼠 血清总胆固醇(TC) 、血清甘油三酯(TG) 、血清高密度脂蛋白(HDL-C) 以及体重和肝、肾、脾重量的影响，并用 VITEK 32 全自动微生物鉴定系统对其进行鉴定。结果显示：筛选得到10 株双歧杆菌；其中菌株4 的体外降胆固 醇能力最高，达57.14% ；它能避免大鼠发胖，降低高脂模型大鼠血清总胆固醇(TC) 、血清甘油三酯(TG) 、大鼠 肝脏内的脂肪存积量和动脉硬化指数，提高大鼠 HDL-C/TC 值；它被鉴定为两歧双歧杆菌(Bifidobacterium bifidum) ， 适于开发安全高效降血脂和预防心血管疾病的保健食品。 ：双歧杆菌；胆固醇；东北虎 Isolation and Identification of Cholesterol-lowering Bifidobacterium bifidum from Northeast Tiger Feces YIN Jun-xia ，SHEN Guo-juan，ZOU Bo ， CHEN Hai-qing ，LI Yang ，DING Zhen-zhen (Department of Biology, Shaoxing University, Shaoxing 312000, China) Abstract：In order to exploit functional food with high efficiency and safety in lowering cholesterol and preventing cardiovascular diseases, the selective medium TPY was adopted to isolate and screen Bifidobacterium from fresh feces of a healthy northeast tiger. The most efficient strain in lowering cholesterol in vitro was administered to artificially induced hyperlipaemia rats by gavage The effects of the strain on serum total cholesterol (TC), triglyceride (TG) and high-density lipoprotein cholesterol (HDL-C) and body and viscera weights of rats were investigated, and the strain was identified by using VITEK-32 automatic microbe analytic systems. The results showed that 10 strains of Bifidobacterium were isolated and screened out in all, among which strain NO. 4 has the highest activity (57. 14%) of lowering cholesterol in vitro. This strain can reduce body weight, serum TC, serum TG, hepatic fat and atherogenic index and enhance HDL-C/ TC value of hyperlipaemia rats. The strain NO.4