用RP_HPLC法测定三黄片中四种组分的含量.pdfVIP

Pharm Care Res 2009 Aug; 9(4) 292 , . RPHPLC RPHPLC 毛泉明, 张钰泉, 叶福媛, 刘新华( , 201203) [ ] 目的: RPHPLC 方法: Kromasil KR1005 C ( 250 mm 4. 6 mm, 5 m) ; : ( A) ! 3%( ) ;: A( %) ! (%) = 23! 77 18 ( 0~ 9 min) , 1 mL/ min, A( %) ! ( %) = 28 ! 72 ( 10~ 21 min) , 0. 7 mL/ min, A (%) ! (%) = 66 ! 34 ( 22~ 35 min) , 1 mL/ min; 270 nm; 35 ∀ 结果: , 97. 00% ~ 102.55% , 结论: , [ ] ; ; ;; ,; [ ] R 284, R 927. 2 [ ] A [ ] 2009) Determination of contents of fourcomponents in Sanhuangtablets by RPHPLCmethod MAO Quanming,ZHANG Yuquan, YE Fuyuan, LIU Xinhua( Laboratory of Analysis and Determination, Center of Science and Technology, Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China) [ABSTRACT] bjective:To establish a RPHPLC method for simultaneous determination of contents of four components in Sanhuang tablets.Methods:HPLC was used to detect the contents of the four components: baicalin, berberine,emodim and chry sophanol. The study w as carried out on a Kromasil KR1005 C column ( 250 mm 4. 6 mm, 5 m) with acetonitrile( A)3% 18 acetic acid w ater solution( ) gradient elution system as mobile phase. A( %) ! (%) was 23! 77 in 09 min w ith flow rate at 1 mL/ min, A(%) ! ( %) = 28! 72 in 1021 min with flow rate at 0. 7 mL/ min and A( %) ! (%) = 66! 34 in 2235 min w ith flow rate at 1 mL/ min. The detective wavelength w as 270 nm and the column temperature w as 35 ∀ .Results:The four compo nent s w ere separated on the baseline.T he average recovery rates w ere in the range of 97. 00%102.55% . The accuracy,repeat ability and stability of the method w ere good.Conclusion:This method is rapid,reliable,simple and applicable. [KEY W RDS] Sanhuang tablets; baicalin; berberine; Rad