实验性糖尿病牙周炎诱导成骨细胞凋亡的研究.docVIP

实验性糖尿病牙周炎诱导成骨细胞凋亡的研究 　　作者：付永伟 和红兵 欧炯光 作者单位：昆明医学院附属口腔医院 口腔内科，云南 昆明 650031 　　【摘要】 目的 观察糖尿病牙周炎条件下成骨细胞的凋亡情况。方法 选用SD大鼠62只，随机分为糖尿病牙周炎组（DP）、单纯牙周炎组（P）以及空白对照组（N）。采用一次性腹腔注射链脲佐菌素（STZ）的方法诱导大鼠糖尿病模型，采用丝线结扎联合口内接种细菌的方法建立牙周炎模型。各组动物分别于DP组、P组丝线结扎后第3、6周时分批处死，取标本进行组织学检测，并计算各组成骨细胞凋亡百分率。结果 丝线结扎后3、6周时，成骨细胞凋亡百分率由高至低依次为DP组、P组、N组，组间两两比较均有统计学意义（P＜0.05）。在丝线结扎后3周和6周时，DP组成骨细胞凋亡百分率均达到P组的2倍。结论 糖尿病可促进牙周炎条件下牙周组织中成骨细胞的凋亡。 　　【关键词】 牙周炎 糖尿病 成骨细胞 凋亡 　　Osteoblast apoptosis in experimental diabetic periodontitis in rats FU Yong-wei, HE Hong-bing, OU Jiong-guang. （Dept. of Oral Medicine, The Affiliated Stomatological Hospital of Kunming Medical College, Kunming 650031, China） 　　[Abstract] Objective To observe osteoblast apoptosis in diabetic periodontitis. Methods Sixty-two SD rats were randomly divided into three groups： Diabetic periodontitis group（DP）, periodontitis group（P） and normal controlgroup（N）. Diabetes was induced by intraperitoneal injection of streptozotocin（STZ）. Periodontitis was initiated byligating floss around maxillary second molars and inoculation of Porphyromonas gingivalis（P.gingivalis）. Three orsix weeks later, the animals were sacrificed and the specimens were prepared for histological analysis. The percentageof apoptotic osteoblasts was examined. Statistical significance was determined by one-way ANOVA. Results The se-quence of percentage of apoptotic osteoblasts among each group was group DP＞group P＞group N. Intergroup comparisons demonstrated that diabetic periodontitis and periodontitis might increase apoptosis of osteoblasts. The percentage of apoptotic osteoblasts in group DP was approximately two times higher than that in group P. Conclusion The present study clearly demonstrates that diabetes can increase the apoptosis of osteoblasts in peri-odontitis. 　　[Key words] periodontitis； diabetes； osteoblast； apoptosis 　　大量临床研究和流行病学调查显示，与非糖尿病患者相比，糖尿病患者牙周炎发病率高，病变损害严重，且进展迅速。糖尿病牙周炎牙槽骨丧失明显增加[1-2]。骨丧失由骨重建失衡所致，骨重建由破骨细胞介导的骨吸收和成骨细胞介导的骨形成协同完成，当调控因素发生改变使得骨吸