基于镉吸附的花生壳酶改性研究.pdfVIP

Doi:10.3969/j.issn.1007-2802.2014.02.008 基于镉吸附的花生壳酶改性研究 1 1,2 1 1,2 赵雅兰 ，易筱筠 ，雷 娟 ，党 志 1.华南理工大学环境与能源学院，广州 510006； 2. 工业聚集区污染控制与生态修复教育部重点实验室，广州 510006 摘 要：本文利用农林废弃物中的花生壳，对其进行生物改性以便获得一种吸附镉的价廉易得的材料。以 漆酶为改性试剂，通过正交实验确定吸附镉的最优改性条件。结果表明在30 ℃，pH为5 时，以100 mL固 定酶活的漆酶溶液对1 g花生壳改性5d效果最佳。相对于改性之前，改性材料的吸附量增加了32% ，2 h后 即趋近吸附平衡。通过红外、XRD 、SEM等表征分析，发现改性材料表面更加粗糙，更多的自由羟基和羧 基得以暴露，这为吸附量的增加提供了依据。在材料投加量为2 g·L-1、溶液浓度为50 mg·L-1时基本达到吸 附饱和。实际废水的浓度大都低于50 mg·L-1 ，因而该改性材料具有一定的实用价值。 关 键 词：花生壳；漆酶；生物改性；吸附 中图分类号：X703 文献标识码：A 文章编号：1007-2802 （2014 ）02- The Research of Cd Sorption By Enzymatic Modified Peanut Hulls 1 1,2 1 1,2 ZHAO Ya-lan ，YI Xiao-yun ，LEI Juan ，DANG Zhi 1. College of Environment and Energy，South China University of Technology ，Guangzhou 510006，China; 2. Key Laboratory of Pollution Control and Ecosystem Restoration in Industry Clusters，Ministry of Education ， Guangzhou 510006，China Abstract ：This article aimed to modify the peanut shell in agricultural and forestry wastes using the biological method to obtain an economic Cd sorption material. Peanut hulls were modified by laccase solutions and the orthogonal experiments were designed to determine the optimal modification conditions of cadmium sorption. The result indicated that the best conditional combination was 30。C, pH 5, and applying 100mL of the fixed enzyme activity of laccase solution to 1g peanut shells for 5d. The sorption quantity was 32% higher than that of unmodified peanut hull, and the sorption could approach equilibrium in 2 hours. The results of FI-IR, XRD, and SEM characterizations showed that