PI法和AnnexinVPI法检测蓝舌病毒HbC3诱导人肝癌细胞的凋亡.pdf

第 21 卷 3 期 中 国 病 毒 学 21 （3 ）：253-256 2006 年 5 月 VIROLOGICA SINICA May 2006 PI 法和 Annexin V/PI 法检测蓝舌病毒 HbC3 诱导 人肝癌细胞的凋亡* 1 1 1 1 2 2** 汪 艳 ，刘春新 ，易有荣 ，崔冶建 ，朱 珺 ，董长垣 （1.武汉大学医学结构生物学研究中心, 湖北武汉 430071 ；2.武汉大学医学院病毒研究所分子病毒与癌研究室，湖北武汉 430071 ） Detection of Apoptosis of the Human Hepatic Carcinoma Cells Induced by Bluetongus Virus by PI and Annexin V/PI Staining Methods 1 1 1 1 2 2** WANG Yan ，LIU Chun-xin ，YI You-rong ，CUI Ye-jian ，ZHU Jun ，DONG Chang-yuan (1.Center of Medical Structural Biology, Wuhan University School of Medicine Wuhan University, wuhan 430071, China; 2.Laboratory of Virus and Cancer, Institute of Virology, Medical College, Wuhan University, Wuhan 430071,China) Abstract ：Flow cytometry was used to quantify the percentage of apoptotic hepatic Caracimoma cells infected with bluetongue virus HbC3 and stained with propidium iodide (PI) and Annexin V/PI. The percentages of the apoptotic cells measured by PI at 24h, 36h and 48h were 10.8 ±3.05, 21.7±6.28, 28.3±10.6, respectively, and those by Annexin V-PI were 20.42±3.70, 49.3±8.11, 79.6±11.5, respectively. The two staining methods and the rate of apoptotic cells infected by bluetengue virus hours were significantly different (P0.01). The early apoptotic cells and secondary necrosis could be detected by AnnexinV/PI assay with good sensitivity and specificity. The apoptotic cells induced by bluetengue v