PCR及其衍生技术在基因突变检测中的应用.pdfVIP

H ER EDI TA S ( B eij ing ) 25( 2) : 198~ 200, 2003 PCR 颜志强, 杨胜利, 龚 毅 ( , 500 200233) : PCR ; PCR , PCR :; ; ; PCR :R37 : A :0253- 9772( 2003) 02- 0198- 03 Use of PCR Related Methods in Detection of Gene Mutation YAN Zh-i Qiang, YANG Sheng- i, GONG Yi ( S hang hai R esearch Ce nter of Biotechnol ogy , I nsti t ute of Biol ogical S ciences , CA S , Shanghai 200233, Chi na) Abstract: Many inherited diseases and drug resistance have been attributed to mutations in corresponding genes. In this pa- per, several techniques based on PCR used in diagnosis were concluded. The development and research progress of Mi- smatch PCR w ere discussed in details.Some informat ion about an assay that w e developed for detection of antimicrobial re- sistance to quinolones w as also described. Key words:inherited diseases; drug resistance; gene mutation; mismatch PCR , : , B- A Barts ( DMD) [1] T ; ddIddC , PCR [2,3] ; 1. ( single strand conformation polymor- [4,5] 80 PCR phism, SSCP) ,,PCR 1.2. 1 原理及应用 [ 6] PCR- SSCP Orita , Jacquemier [ 7] PCR DNA , (PAGE)