不明病原体高通量快速筛查的生物芯片技术.pdfVIP

Huang et al. BMC Infectious Diseases 2013, 13:437 /1471-2334/13/437 TECHNICAL ADVANCE Open Access An easy operating pathogen microarray (EOPM) platform for rapid screening of vertebrate pathogens 1† 2† 3 4 4 4 5 Weiwei Huang , Yinhui Yang , Xinlei Zhang , Changan Zhao , Aihua Yin , Xiaozhuang Zhang , Zhengxin He , Yongqiang Jiang2* and Liang Zhang1* Abstract Background: Infectious diseases emerge frequently in China, partly because of its large and highly mobile population. Therefore, a rapid and cost-effective pathogen screening method with broad coverage is required for prevention and control of infectious diseases. The availability of a large number of microbial genome sequences generated by conventional Sanger sequencing and next generation sequencing has enabled the development of a high-throughput high-density microarray platform for rapid large-scale screening of vertebrate pathogens. Methods: An easy operating pathogen microarray (EOPM) was designed to detect almost all known pathogens and related species based on their genomic sequences. For effective identification of pathogens from EOPM data, a statistical enrichment algorithm has been proposed, and further implemented in a user-friendly web-based interface. Results: Using multiple probes designed to specifically detect a microbial genus or species, EOPM can correctly identify known pathogens at the species or genus level in blinded testing. Despite a lower sensitivity than PCR, EOPM is sufficiently sensitive to detect the predominant pathogens causing clinical symptoms. During application in two recent clinical infectious disease outbreaks in China, EOPM successfully identified the responsible pathogens. Conclusions: