_南极假丝酵母生产脂肪酶发酵条件研究副本.pdfVIP

第26 卷第2 期 青 岛 科 技 大 学 学 报 V ol. 26 No. 2 2005 年4 月 Journal of Qingdao University of Science and T echnology A r. 2005 : 1672-6987(2005) 02-0101-05 , , , , , (青岛科技大学 化工学院, 山东青岛 266042) : 用南极假丝酵母( C. ant ar ct ic a DM S-3855) 发酵生产胞外脂肪酶。通过摇瓶和 15 L 发酵罐发酵实验, 研究了培养基成分和 作条件, 得到较优的培养基组成和发酵生 产的工艺条件。在培养基组成: 豆粉40 g # L- 1, 淀粉 15 g # L- 1 , 豆油5 mL # L- 1 等; 作条件: 温度24 e , 初始 H61 0, 通气量 1010 L # min- 1, 发酵周期缩短到54 h 。由15 L 发酵罐生产酶液的酶活达到1912 U # mL- 1 。 : 发酵; 南极假丝酵母; 脂肪酶 : Q 93 : A Studies on Fermentation Process for Lipase Production by Cand ida a ntarct ica LIU Jun-hong, XU Jun-wei, WA G Fan-ye, WU Ru-lin, YA G Feng-ke, A Jun-long ( College of Chemical Engineering, Qingdao University of Science and T echnology, Qingdao 266042, China) Abstract: The roduction of extracellular li ase from Cand id a ant ar ct ica w as studied in both shake-flask and 15 L fermenter. T he nutrient medium com osition and the o era- tion conditions were o timized to obtain the o timal nutrient medium and the o timal o era- - 1 tion conditions. Under the nutrient medium com osition of bean owder 40 g # L , starch 15 - 1 - 1 g # L , bean oil 5 mL # L and the o eration conditions of tem erature 24 e , H6. 0, - 1 gas amount 1010 L # min , the eriod of fermentation w as reduced to 54 h. T he maxi- mum li ase yield of 1912 U # mL- 1w as achieved in 15 L fermenter. Key words: fermentation; C. ant ar ct ica ; li ase 脂肪酶以其来源广、催化功能多以 催化底 1 实验部分 物广泛的优势成