刘义涛论文.doc

2011届 　　 　　 　　　　　 分类号： 　　　　　　　　　　　　　　　　　 单位代码 ：10452 毕业论文 槐米中芦丁超声法和碱溶酸沉法提取比较 姓 名　 刘义涛 学 号　 200709820229 年 级　 2007级 专 业　　 制药工程 系　（院）　 生命科学学院 指导教师　 张海娟 二〇一一年五月 槐米中芦丁超声法和碱溶酸沉法提取比较 Comparation of Ultrasonic and Alkali solution and acid precipitation extracted Rutin from Sophora japonica ·1 姓 名 刘义涛 专 业 制药工程 　　　　　　　 指导教师 张海娟 临沂大学 二年月 槐花中,又名芸香苷, ABSTRACT This experiment compared ultrasonic extraction with alkali solution and acid precipitation in extraction rate of rutin. Ultrasonic method is soaked Sophora japonica with 70% ethanol, the ultrasonic cleaner carried out the Sophora japonica with 30 minutes .then filtering the solution and carried out the residue in the ultrasonic cleaner again. Collecting the filtrate in beaker and Adjusting the filter’s PH to 3 with concentrated hydrochloric acid. Standing the filter and precipitating completely. Collecting the precipitate by the filtration. The precipitate is crude extract rutin. Recrystallizating the crude extract rutin in 60-70 ℃ water bath and boiling the recrystallizate’s solution on electric furnace with 15 minutes. Filtrating the solution when it is boiling and the filtrate aside until the precipitate completely. Filtrating the solution, Then collecting the precipitate. The precipitate dried at 65 ℃ in Vacuum oven with40 minutes . Getting the purified rutin. Alkali solution and acid precipitation is soaked Sophora japonica with distilled water, Adding calcium oxide 1.5 g. adjusting the PH to 8, Boiling the Solution with 30 minutes, then filtering the solution and carried out the residue again, Collecting the filtrate in beaker and Adjusting the filter’s PH to 5 with concentrated hydrochloric acid. Standing the filter and precipitating completely. Collecting the precipitate by the filtration. The precipitate is crude extract rutin. Recrystallizating the crude ext