2000-中国林业研究-盐胁迫下胡杨与毛白杨悬浮细胞的离子区隔化(英文).pdfVIP

8 Cellular Distribution of Ions in Saltstressed Cells of Populus euphratica and P . tomentosa Chen Shaoliang 　Eberhard Fritz 　Wang Shasheng Aloys Hüttermann 　Liu Qunlu 　Jiang Xiangning ABSTRACT 　The authors used suspension cells of a saltresistant poplar genotype Popul us euphratica and a saltsensitive genotype P . tomentosa to examine genotypic differences in intracellular compartmen tation of ions under NaCl stress. Cells of P . euphratica were treated with 52 , 137 , 223 , 309 , 395 and - 1 - 1 480 mmol ·L NaCl respectively and P . tomentosa was salinized with 137 mmol ·L NaCl. Spot energy dispersive Xray microanalyses showed that salt treatment significantly increased Na and Cl levels in cell compartments : cell wall , cytoplasm and vacuole. Preferential accumulation of Cl relative to Na in cyto plasm and vacuole indicates that Na and Cl may translocate independently from apoplast to symplast . Compared with P . tomentosa , P . euphratica accumulated much less salt ions in cytoplasm at 137 mmol - 1 ·L NaCl. Furthermore , with increasing salinity , Na and Cl levels in cytoplasm of P . euphratica were - 1 remained lower than that in walls when NaCl below 223 mmol ·L . Collec