FK506和神经干细胞对异体神经移植生长的作用.pdfVIP

·1384· ·实验研究· FK506及神经干细胞对异体神经移植生长 的作用 李明新 阚世廉张建国 李瑞华 【摘要】 目的探讨大鼠坐骨神经异体神经移植后联合应用FK506(他克莫司)及神经干细胞 (NSCs)的可行性。方法制造大鼠坐骨神经缺损模型，异体神经移植修复；根据分组不施加干预因 素、吻合口注入NSCs、腹腔注射FK506，通过运动及感觉评分、电生理检测、组织学检查，以及免疫组 织化学染色，观察神经再生差异。结果 同时应用FK506及NSCs组，感觉及运动评分(5．32± 1．43)，神经传导速度(20．52±1．45)m／s，GAP-43染色(965．724-369．17)都明显优于其他组(P 0．05)。结论FK506结合NSCs对异体神经移植生长具有促进作用。 【关键词】 FK506；神经干细胞；异体神经移植 ofF]娜incombinationwithneuralstemcells the in Experimentalstudy improveregeneration nerve Traumatic allografts廿Ming-xin，KAN Shi—lian，ZHANG胁酗guo，“Rui—hua．Departmentof Surgery，TianjinHospitalTianjinshi，300211，China author：LI CorrespondingMing-xin，Email：limingxin5@126．COIn 111is theeffectofFK506andneural 【Abstract】0bjectiveexperimentstudy stemceUsonthe渺 innerve AnestablishedratsciaticnervemodelWaSused．Sciaticnerve generation allografts．Methods werecreatedintheLewisrats．Nerves wereharvestedfromBNratsto the (10mill)deftcits allografts repair deficitsoftheLewis Areceivedno Breceivedneuralstem Dre． mts．Group treatment．Group cells．Group ceived CreceivebothneuralstemeelsandFK506．Nerve wasevaluated FK506．Group regeneration by standardizedand tests．Nerve and muscleswereharvestedfor pin—pricktoe．spread samplesgastrocnemiUS examination．Muscledenervationwasevaluated weights．GAP-43andBrdU(Two． atrophy bygastrocnemiUS detection test．ResultsIm． stepimmunohistochemistryreagent)wereassayedbyimmunohistochemistry functionaloutcomeswereobservedin Cwhen withother conduction proved