血管紧张素Ⅱ上调自发性高血压大鼠和Wistar--Kyoto大鼠血管平滑肌细胞外信号调节激酶的信号转导.pdfVIP

Acta Physiologica Sinica, October 25, 2005, 57 (5): 587-592 587 Wistar-Kyoto * 310003 (angiotensin Ang )(spontaneously hypertensive ratSHR)Wistar- Kyoto (WKY)(vascular smooth muscle cells, VSMCs)(extracellular signal-regulated pro- 5 tein kinases ERKs) SHR WKY VSMCs 1×10 mmol/L 1×105 mmol/L PD98059 1×107 mmol/L Ang 24 h VSMCs ERK Western-blot ERK (total ERK t-ERK)ERK (phosphorylated-ERKp-ERK)-1 (mitogen-activated protein kinases phosphatase-1, MKP-1) RT-PCR MKP-1 mRNA (1) SHR WKY Ang VSMCs ERK p-ERK MKP-1 MKP-1 mRNA (P 0.05); SHR WKY Ang Ang +PD98059 (2) SHR VSMCs ERK p-ERKMKP-1 MKP-1 mRNA WKY (P0.01) (3) SHR WKY Ang Ang + Ang +PD98059 VSMCs t-ERK Ang 1 (Ang type 1 AT 1)SHR WKY VSMCs ERK ERK p-ERK MKP-1 MKP-1 mRNA Q254; R544.1 Effects of angiotensin on extracellular signal-regulated protein kinases signaling pathway in cultured vascular smooth muscle cells from Wistar-Kyoto rats and spontaneously hypertensive rats * ZHU Jian-Hua, LIU Zhong, HUANG Zhao-Yang , LI Shan Department of Cardiology, the First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China Abstract: The aim of this study was to investigate the effects of angiotensin (Ang ) on extracellular signal-regulated protein kinase (ERK) signaling pathway in cultured vascular smooth muscle cells (VSMCs) from spontaneously hypertensive rats (SHR) and Wistar-