生物过程—新型假单胞菌对羟基苯乙腈水解酶及其性质研究.pdfVIP

Bioprocess 生物过程, 2012, 2, 70-74 doi:10.4236/bp.2012.22012 Published Online June 2012 (/journal/bp) A New 4-Hydroxyphenylcyanide Nitrilases from Pseudomonas * Sp. and Its Catalytic Properties 1 2 2# 2 2 2 Mingle Cao , Xinglin Jiang , Haibo Zhang , Mo Xian , Xin Xu , Wei Liu 1State Key Laboratory of Microbial Technology, Shandong University, Ji’nan 2Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao Email: #zhanghb@ Received: May 13th, 2012; revised: May 27th, 2012; accepted: Jun. 4th, 2012 Abstract: Nitrilases can hydrolyze nitrile efficiently under mild conditions. The enzymatic methods have the advan- tages of less pollution, low cost compared with the chemical methods. Nitrilases are potentially applied in agriculture, industry, environment, and biomedicine. In this study, Berthelot method and high performance liquid chromatography were used to screen new stains for nitrilases. A nitrilase with high substrate specificity for 4-hydroxyphenylcyanide was found from Pseudomonas sp. 6-1. The stain produced 28.47 U/ml nitrilase after optimization of the culture conditions. The enzyme remained 80% activities at pH 6.6 to pH 7.6, temperature 35˚C to 45˚C, and it was stable after 18 h incuba- tion. The stain can be potentially used in biosynthesis 4-Hydroxyphenylacetic acid using 4-hydroxyphenylcyanide as substrate. Keywords: 4-Hydroxyphenylcyanide; 4-Hydroxyphenylacetic Acid; Nitrilase; Biocatalysis 新型假单胞菌对羟基苯乙腈水解酶及其性质研究* 1 2 2# 2 2 2 曹明乐 ，姜兴林 ，张海波 ，咸 漠 ，徐 鑫 ，刘 炜