真核表达系统的研究进展_高云.pdfVIP

·292 · 8 　4 　　　　 Vol.8　No.4 2002 8 National Journal of Andrology Aug.2002 · · 高　云　;黄宇烽　 (, 210002) :, 。 :、。 , , 。 , ;- S 。 , 2 , Cre/loxp , ; , ;, 、、。 :;;; :Q813　　:A　　:1009-3591 (2002)04-0292-03 Advances in Eukaryotic Expression Sy stems Yun AO, Yu-Feng HUAN (Laboratory of Reproduction Genetics, Nanj ing General Hosp ital of Nanj ing Command, PLA , Nanj ing, J iangsu 210002, China ) Abstract:The increasing popularity of eukaryotic expression systems can be attributed to their capability of performing many post- translational modifications.At present, There mainly are three expression systems including yeast expression system, insect cell ex- pression system and mammalian cell expression system.The methylotropic yeast Pichia Pastoris usually utilizes alcohol oxidase pro- moter to drive the expression of foreign gene.Recently, a continous fermentation has been developed in PichiaPastoris with the glyc- eraldehyde-3-phosphate dehydrogenase (AP)promoter.The baculovirus-mediated insect cell expression system is considered to be safe, powerful, but cell-lytic.Baculovirus-S system uses the popular and genetically well understood Drosphila S cells which do not 2 2 appear to be lysed after infection.In mammlian cell expression system, recombinant adenovirus are attracting a great deal of attention as a highly efficient gene transfer vehicle.Thefrequency of Ad vector rescue by homologous recombination in E.coli and Cre-medi- ated site-specific recombination is significantly higher thanby homologous recombination in vivo.Tetracycline-regulatable system is awidely used mammalian cell