电离辐射对小鼠腹腔巨噬细胞IL_12和NF_B活性的影响.pdfVIP

2003 2 23 1 Chin J RadiolMedProt, February 2003,Vol23,No. 1 7 IL12NFB 刘晓冬 马淑梅 刘树铮 ! IL12 NFB (ELISA) IL12 , (EMSA) NFB ∀ ELISA , IL120075 Gy X 4~ 8 h (P 005) ,2 Gy2 h , 48h (P 005) ,2 GyIL12 0075 Gy; # EMSA ,NFBP65P50P50P50 ,0075 Gy P65P50 ,200 (2~ 8h), P50P50 ; 2 Gy P65 P50P50P50 ,2~ 8 h200 IL12 NFB NFB IL12 ! ; IL12; NFB; ; Effects of ionizing radiation on IL12 andNFB activities in peritoneal macrophages of mice LI UX iao dong ,MA Shumei ,LI U Shuz heng .MH Radiobiology R esearch Unit ,Jilin Univ ersity , Chang chun 130021,China Abstract! Objective Toobservethe changes of IL12 andNFB in murine peritonealmacrophages af terwholebody irradiation(WBI) with different doses Xrays. Methods Enzymelinked immunosorbent assay ( ELISA) and electrophoresismobility shift assay(EMSA) wereused to detect the secretionof IL12 and theDNA binding activity of NFB,respectively. esults ELISA data indicated both 0075 Gy and 2 Gy increased the secretion of IL12 and the latter seemed to increase IL12 more greaterthan theformer.EMSA showed two bands representing P65P50 and P50P50 respectively. 0075 Gy increasedtheP65P50 activityand showedlittleeffect on P50P50,while2 Gy increasedboth thetwo dimmers, consequently elevatedtheDNAbinding activityof NFB moresignificantly. Conclusion The results suggested that NFB might participate in the radiationinduced changes of IL12. Key words! Ionizing radiation; IL12; NFB; ELISA; EMSA [35] IL12 , IL12 NFB,NF p35 p40 , BIL12 T NK ,T NK , Th1 1: ,,(20∃ 2)g,, [ 1] , p35 (