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a novel method for the measurement of hepatitis c virus infectious titres using the incucyte zoom and its application to antiviral screening论文.pdf

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Journal of Virological Methods 218 (2015) 59–65 Contents lists available at ScienceDirect Journal of Virological Methods j o u r n a l h o me p a g e : w w w. elsev /locate/jviromet A novel method for the measurement of hepatitis C virus infectious titres using the IncuCyte ZOOM and its application to antiviral screening Hazel Stewart a , Christopher Bartlett a , Douglas Ross-Thriepland a , Joseph Shaw b , Stephen Grifﬁn b , Mark Harris a,∗ a School of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, United Kingdom b Leeds Institute of Cancer and Pathology, Faculty of Medicine and Health, St James’s University Hospital, University of Leeds, Leeds LS9 7TF, United Kingdom s u m m a r y Article history: Hepatitis C virus (HCV) is a signiﬁcant human pathogen infecting 3% of the world population. An infec- Received 1 December 2014 tious molecular clone capable of replicating and releasing infectious virions in cell culture has only been Received in revised form 11 March 2015 available since 2005, leaving a signiﬁcant knowledge gap concerning post-RNA replication events such Accepted 12 March 2015 as particle assembly, trafﬁcking and release. Thus, a fast, efﬁcient and accurate method of measuring Available online 20 March 2015 infectious viral titres is highly desirable. Current methods rely upon manual counting of infected cell foci and so are both labour-intensive and susceptible