四种仿生骨组织工程支架材料的细胞相容性.pdfVIP

12 27 Journal of Clinical Rehabilitative Tissue Engineering Research July 1, 2008 Vol.12, No.27 * 1 2 1 1 1 1 Compatibility of osteoblasts on four kinds of biomimetic composite scaffolds of bone tissue engineering 1 2 1 1 1 1 Zhang Mei-xia , Yang Chun-rong , Yao Yao , Min Shao-xiong , Li Miao , Jin An-min Abstract 1Department of Hematology, BACKGROUND: In vitro cell culture in the biomimetic physiological condition is a convenient method to prevent the biomaterials Zhujiang Hospital, Southern Medical from multiple factors in vivo, and to control the experimental conditions with potent reproducibility. University, OBJECTIVE: To explore the compatibility of osteoblasts culturing on four kinds of biomimetic composite scaffolds, which are Guangzhou prepared modified bioactive glass (BG), collagen (COL), hyaluronic acid (HYA) and phosphoserine (PS). 510282, Guangdong Province, China; DESIGN, TIME AND SETTING: The controlled experiment was conducted in the Clinical Medicine Research Institute of 2 School of Material Zhujiang Hospital, Southern Medical University (Guangzhou, Guangdong, China) from March to August in 2006. Sciences and MATERIALS AND METHODS: Mouse embryo osteoblasts were offered by the Department of Pathogenic Biology in Zhejiang Engineering, South China University of University Medical College. Four kinds of biomimetic three-dimensional composite scaffolds of bone tissue engineering were Science and prepared by means of freeze drying and biomimetic mineralization. Mouse embryo osteoblasts were seeded on the four kinds