Stathmin在宫颈癌中的表达及意义.pdfVIP

2009 12 18 12 ProgObstetGyneco,l Dec. 2009, Vol18, No12 Stathmin * 叶丽华,杜亚丽 ,姜海燕 (泰州市人民医院妇产科,泰州 225300) :探讨 Stathm in/oncoprotein 18(Op18)蛋白在宫颈癌的表达及意 : 应用免疫组化和Western blot法检测 15例正常宫颈组织26例宫颈上皮内瘤变组织 和 52例宫颈癌组织中Stathmin蛋白的表达: 用免疫组化检测 Stathmin蛋白在正常 宫颈组织宫颈上皮内瘤变及宫颈癌的阳性表达率分别为 20. 00 53. 85 84. 62 正常宫颈组织组分别与宫颈上皮内瘤变及宫颈癌组比较, 差异均有统计学意 (P 0. 05); 宫颈上皮内瘤变组与宫颈癌组比较, 差异有统计学意 (P 0. 05)Western blot 法检测显示,正常宫颈组分别与宫颈上皮内瘤变组宫颈鳞癌组及宫颈腺癌组比较, 差异 均有统计学意 (P 0. 01), 宫颈上皮内瘤变组分别与宫颈鳞癌组及宫颈腺癌组比较, 差 异均有统计学意 (P 0. 01)Stathmin与宫颈癌的临床分期组织分级区域淋巴结转 移有关: Stathmin在宫颈癌过表达,与宫颈癌的发生发展密切相关 宫颈肿瘤; Stathmin, 癌基因蛋白类; 免疫组织化学;蛋白质印迹法 : R735. 1 : A : 1004- 7379( 2009) 12- 0902- 04 Expression and significance of Stathm in in cervical carcinomas. Ye L ihua, Du Yali, J iang H aiyan. D ep artment of Obstetrics and Gynecology, Ta izhou P eop le sH osp ital, Taizhou 225300 Abstract Objective: To explore the expression and significance ofStathmin/oncopro- tein 18(Op18) in cervical carcinomas.M ethods: Fifty-two cases of cervical carcinoma, 15 ca- sesofnormal cervical tissues and 26 caseswith CIN were examined by mi munohistochem ical staining andWestern blotmethod. Results: The mi munohistochem ical analysisdemonstrated that the positive rates of Stathmin in normal cervical tissue group, CIN and cervical carcinoma groupswere20. 00 , 53. 85 and 84. 62 , respectively.The difference among cervicalcarc-i noma, CIN andnormal cervical tissuegroupswas significant(P 0. 05). Thedifferencebewt een cervical carcinoma andCIN groupswas significant(P 0. 05).The expression levelofStathmin in cervical carcinomawashigher than that of normal cervical tissue group. Therewere signif-i cant differences among normal cervical group and CIN, cervical squamous carcinoma, cervical adencarcinoma groups(P 0. 01), respectively.The differences among CIN and cervical ca