分光光度计法测定甲基红离解常数 实验数据处理.docVIP

V、Data treatment Part 1：Measure the maximum absorption Table 1: Absorption of solution A Wavelength/nm absorption 420 0.075 440 0.172 460 0.41 480 0.762 500 1.14 510 1.286 520 1.353 530 1.332 540 1.263 560 0.905 580 0.295 600 0.046 Graph 1: Absorption of solution A Conclusion：from the Graph 1，we can get the wavelength for the maximum absorption of solution A is 520 nm. Table 2: Absorption of solution B Wavelength/nm absorption 410 0.52 420 0.543 430 0.552 440 0.552 450 0.54 460 0.498 480 0.354 500 0.178 520 0.074 540 0.037 Graph 2: Absorption of solution B Conclusion：from the Graph 2，we can get the wavelength for the maximum absorption of solution A is 430-440 nm. Part 2：Test the Beer’s law Table 3: Absorption of HMR in different concentrations No.(concentration) Absorption (520nm ) Absorption (430nm ) 0#-c(A) 1.353 0.147 1#-0.75c(A) 1.064 0.106 2#-0.5c(A) 0.68 0.064 3#-0.2c(A) 0.373 0.055 Note：because it is always acidic through the process, we can ignore the absorotion of MR-. Graph 3：Absorption of HMR in different concentrations（520nm ) (the forth data is ignored due to its large derivation) Graph 4：Absorption of HMR in different concentrations（430nm ) Conclusion：(C0=1.851*10-5) from the Graph 3，the slope is 1.2510, so §HNM520=1.2510/(C0=1.851*10-5)=6.79*104，from the Graph 4，the slope is 0.166, so §HNM430=0.166/(C0=1.851*10-5)=8.97*103 . Table 4: Absorption of MR- of different concentrations No.(concentration) Absorption (520nm ) Absorption (430nm ) 0#-c(B) 0.074 0.552 1#-0.75c(B) 0.0455 0.288 2#-0.5c(B) 0.0479 0.134 3#-0.2c(B) 0.0322 0.064 Note：because it is alkaline through the process, we can ignore the absorotion of HMR. (the second data is ignored due to its large derivation) Graph 5：Absorption of MR- in different concentrations（520nm ) (the forth data is ignored due to its large derivation) Graph 6：Absorption of MR- in different concentrations（430nm ) Conclusion：(C0=1.851*10-5) from the Graph 5，the slope is