ATP荧光检测法与细菌定量检测法现场细菌污染检测相关性研究.pdfVIP

中国消毒学杂志2014 年第31 卷第12 期 ·1285 · 【论著】 ATP 荧光检测法与细菌定量检测法 现场细菌污染检测相关性研究 1 王绍鑫，周艳琴，罗剑峰 ，李凌雁，成 鳭 (上海市卫生局卫生监督所，上海200031; 1 复旦大学) 摘要 目的 研究ATP 生物荧光检测法与细菌定量检测法对现场环境物体表面细菌负载检测结果相关性及数学 模型。方法 通过现场采样与检测方法，对两种检测技术结果进行统计分析和评价。结果 ATP 荧光检测法与常 规细菌定量检测法检测结果Peason 相关系数为0. 851，二次方模型y = 0. 804 － 0. 095x + 0. 309 x2 (Ｒ = 0. 785 )拟合 度较高。结论 ATP 荧光检测法与常规细菌定量检测法在现场物体表面细菌负载检测中结果具有较好相关性，其 检测结果具有一定的可靠性。 关键词 ATP 生物荧光法;细菌定量检测法;细菌污染;相关性 中图分类号:Ｒ181. 2 文献标识码:A 文章编号:1001 － 7658 (2014)12 － 1285 － 03 STUDY ON COＲＲELATION OF BACTEＲIAL CONTAMINATION DETECTION ON THE SPOT OF QUANTITATIVE METHOD AND ATP FLUOＲESCENCE ASSAY WANG Shao － xin ，ZHOU Yan － qin ，L UO Jian －f eng 1 ，Li Ling － yan ，CHENG Min (Shanghai Municipal Health Bureau ，Agency for Public Health Inspection ，Shanghai 200031 ;1 Fudan University ，China) Abstract Objective To study the correlation and the mathematic model of ATP fluorescence assay and bacterial quanti- tative detection method for the bacteria load on the object surface on the spot. Methods The field sampling and detection methods were used to carry out statistical analysis and evaluation of the two detection results. Ｒesults The Peason correla- tion coefficient of ATP fluorescence test and result of bacteria counting method was 0. 851. The fitting degree quadratic mod- el y = 0. 804 － 0. 095x + 0. 309 x2 (Ｒ = 0. 785)was higher. Conclusion Ｒesults of the ATP fluorescence assay and the lab bacteria counting method for the bacteria load on the object surface on the spot have a good correlation. Ｒesults Ｒe- sults of these detections are reliable. Key words A TP bioluminesce