常见中草药对鳖源致病性嗜水气单胞菌的体外抑制效应【毕业论文】.doc

本科毕业论文 （20 届） 常见中草药对鳖源致病性嗜水气单胞菌的体外抑制效应 专业：水产养殖学  目录 中文摘要…………………………………………………………………………… 1 英文摘要…………………………………………………………………………… 2 1.前言……………………………………………………………………………… 3 2.材料与方法 ……………………………………………………………………4 2.1实验材料 …………………………………………………………… 4 2.2实验方法 ……………………………………………………………………5 3.结果与分析 …………………………………………………………………… 5 3.1最小抑菌浓度测定结果………………………………………………………6 3.2最小杀菌浓度测定结果………………………………………………………6 4.讨论 ……………………………………………………………………………… 6 4.1实验中草药药性分析…………………………………………………………7 4.2最小抑菌浓度和杀菌浓度的对比分析………………………………………8 致谢 ……………………………………………………………………………8 参考文献 …………………………………………………………………………… 9 常见中草药对鳖源嗜水气单胞菌的体外抑制效应 [摘要] 以“廉价、易得、广谱”为筛选原则，选取其中对革兰氏阴性杆菌具杀灭效果的鱼腥草、黄连、黄芩、金银花、连翘、穿心莲、大青叶、蒲公英、板蓝根等9种常见廉价中草药的10倍水提粉剂（按中草药原料重量的10倍浓缩，即10克中草药浓缩成1克的水提粉剂）作为实验药物。通过对最小抑菌浓度和最小杀菌浓度的测定，从中筛选出效果比较好的中草药。结果：黄芩、金银花、蒲公英对嗜水气单胞菌抑菌作用较明显,其MIC≤12.50mg/mL,其次是穿心莲、黄连,其MIC均为12.50mg/mL, 鱼腥草、连翘、大青叶、板蓝根等其他4味药的MIC均≥12.50 mg/mL。得到9种中草药的抑菌效果从高到低依次为黄芩、金银花、蒲公英、连翘、穿心莲、黄连、鱼腥草、板蓝根和大青叶。杀菌效果和抑菌效果相似。 [关键词] 中草药 嗜水气单胞菌 最小抑菌浓度 最小杀菌浓度  Common herbal source of turtle Aeromonas hydrophila in vitro inhibitory effect [Abstract] To cheap, easy to get, broad-spectrumfor the screening principles, select one of the killing effect of gram-negative bacilli with Heartleaf Houttuymia Herb, Coptis Root, Baical Skullcap Root, Honeysuckle Flower, Forsythia, Common Andrographis Herb, Indigowoad Leaf, Mongolian Dandelion Herb, Indigowoad Root,Cheap common herbs such as 9 to 10 times the water extract powder (herbal raw materials by 10 times the weight of concentrated herbal concentrate that is 10 grams to 1 gram of water extract powder) as the experimental drug. By the minimum inhibitory concentration and minimum bactericidal concentration determination, better results from selected herbs. Results: Baical Skullcap Root, Honeysuckle Flower，Mongolian Dandelion Herb of Aeromonas hydrophila obvious inhibitory effect, the MIC ≤ 12.50mg/mL, followed by Common Andrographis Herb, Coptis Root, the MIC are 12.50mg/mL, Heartleaf Houttuymia Herb, Forsythia,