四羟基二苯乙烯苷对慢性脑缺血致痴呆模型大鼠保护作用_刘丽.pdfVIP

1,2 1 1 1 1 1* 刘丽 ,赵玲 , 李雅莉 ,张丽,叶翠飞 , 李林 ( 11 ,, 100053;21 ,, 100176) : 检测慢性脑缺血模型大鼠学习记忆能力的改 海马神经元的损伤和脑内过氧化水平的改 , 并观察何首乌成 分四羟基二苯乙烯苷(2,3,5,4c-tetrahydroxystilbene-2- O-B-D-glucoside,TSG)在上述各方面是否具有显著的改善和保护作用 雄性SD 大鼠双侧颈总动脉永久性结扎制备慢性脑缺血致痴呆模型采用Morris 水迷宫和避暗实验检测大鼠的学习记忆 能力,用尼氏染色方法检测海马神经元细胞, 采用生化方法检测皮层及海马脂质过氧化物丙二醛(MDA) 含量和谷胱甘肽过氧 化物酶( GSH-PX) 活性 慢性脑缺血模型组大鼠空间学习记忆能力和被动回避学习记忆能力较假手术对照组明显降 低,海马神经元细胞减少, 尼氏体显著减少,皮层和海马MDA含量较假手术对照组明显升高, GSH-PX 活性显著降低TSG 灌 胃给药可显著改善模型动物的空间和被动回避学习记忆能力, 减轻脑缺血后海马神经元的损伤, 增强皮层和海马GSH-PX 活 性,降低脑组织MDA含量 TSG 可改善双侧颈总动脉结扎致痴呆模型大鼠的学习记忆能力,减轻海马神经元的损伤, 提高脑内抗氧化能力,降低其过氧化水平 : 四羟基二苯乙烯苷; 何首乌;慢性脑缺血; 痴呆;学习记忆;海马神经元; 脂质过氧化;抗氧化酶 :R965 :A : 1001- 2494( 2006) 05- 0354- 05 Protective Effect of 2, 3, 5, 4c-Tetrahydroxystilbene-2- B-O-D-glucoside on Hippocampal eurons in Dementia Rats Induced by Chronic Cerebral Ischemia 1, 2 1 1 1 1 1* LIU Li , ZHAO Ling ,LIYa-li ,ZHANGLi ,YE Cui-fei ,LIlin ( 11Department of Pharmacology, XuanwuHosp ital of Capital University of Medical ciences, Beij ing 100053, China; 21National Institutef or the Control of Pharmaceutical and Biological Products, Beij ing 100176, China) ABSTRACT: OBJECTIVE To detect the changes in learning and memory ability, the injury of hippocampal neurons and the intracephalic peroxidation level in model rats with chronic cerebral ischemia, and to investigate whether 2,3,5,4c-tetrahydroxystilbene-2- O-B-D-glucoside (TSG), the main effective component of polygonum multiflorum, has significant improving and protecting action in the above as- pects1METHODS The chronic cerebral ischemiamodels were induced by the 8-week permanent ligation of bilateral carotid arteries in male SD rats1 Morriswater mazeandthestep-through testwere usedtodetect thelearningandmemory ability1 Nissl. s stainingwasused to show the status of hippocampal neuron