水稻类钙调磷酸酶亚基B蛋白质在逆境胁迫下表达.pdfVIP

中国农业科学 2013,46(1): 1-8 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2013.01.001 水稻类钙调磷酸酶亚基B 蛋白质在逆境胁迫下的表达 贾 霖，刘雨萌，范 伟，关明俐，贾 盟，窦世娟，魏 健，彭业博，刘丽娟，李莉云，刘国振 （河北农业大学生命科学学院，河北保定071001 ） 摘要：【目的】了解水稻CBL 蛋白质在逆境胁迫下的表达模式进而探讨水稻耐逆的分子机理。【方法】采用基 于抗体的蛋白质组学策略，以超级杂交稻父本9311 为材料，采用免疫印迹（western blotting，WB）技术调查了 CBL 家族主要成员在冷、热、旱、淹和盐胁迫等5 种逆境中的表达特征。【结果】发现CBL1、CBL5 和CBL6 在冷胁 迫中表达下调,CBL1 和CBL2 在热胁迫中表达下调，CBL1 和CBL5 在旱胁迫中表现下调，而CBL6、CBL8 和CBL10 在 旱胁迫中表现上调，CBL1、CBL5、CBL6、CBL8 和CBL10 在淹涝胁迫中表达上调，而CBL2 表现下调。【结论】鉴定 了水稻在重要逆境胁迫下发生丰度变化的6 个CBL 蛋白质。 关键词：水稻；免疫印迹；逆境胁迫；CBL；蛋白质表达 The Expression Profiling of Rice Calcineurin B-Like Proteins in Seedlings Under Environmental Stresses JIA Lin, LIU Yu-meng, FAN Wei, GUAN Ming-li, JIA Meng, DOU Shi-juan, WEI Jian, PENG Ye-bo, LIU Li-juan, L I Li-yun, LIU Guo-zhen (College of Life Sciences, Agricultural University of Hebei, Baoding 071001, Hebei) Abstract: 【Objective 】 The objective of this experiment is to investigate the expression profiling of rice calcineurin B-like (CBL) proteins and the mechanism of stress-tolerance. 【Method 】The expression patterns of CBL proteins at seedling stage of super hybrid rice parental line 9311 under cold, hot, drought, submerge and salt stresses were surveyed by Western blotting (WB) via antibody-based proteomics strategy. 【Result 】The results indicated that the expressions of CBL1, CBL5 and CBL6 were down-regulated under cold stress, the expressions of CBL1 and CBL2 were down-regulated under hot stress, the expressions of CBL1 and CBL5 were down-regulated while CBL6, CBL8 and CBL10 were up-regulated under drought stress. The expressions of CBL1, CBL5, CBL6, CBL8 and CBL1