柑橘溃疡病菌抗铜相关基因copA和copB克隆及原核表达.pdfVIP

A CTA PHYTOPATHOLOG ICA SIN ICA 41( 3): 247-252( 2011) c pA c pB 1 1 1 1, 2 1, 2* 王 君, 叶 刚 , 马燕侠 , 王国平 , 洪 霓 1 ( , 430070; 2 , 430070) : DNA , c pA c pB PCR , 1 782 bp 1 095 bp 2 ( pET-c pA pET-c pB ), [E scherichia c li BL2 1( DE3) ] , c pA c pB EL ISA 1B6 400 c pA c pB W estern bl t, , : ; PCR; ; W estern bl t; C l n ing and p r kary t ic ex p ress i n f the c pp er-re s is tan ce re lated gene s c pA and c pB f X an th m na s citri subsp. c itri 1 1 1 W ANG Jun , YE G ang , M A Y an-x ia , 1, 2 1, 2 1 W ANG G u -p ing , HONG N i ( C llege f Plant Science and T echn l gy, Huazh ng A gricu lturalUn iversity, K ey Lab f C r p D isease M n it ring and Safety C ntr l in H ube,i W uhan 430070, Ch ina; 2H uazh ng Agricultural Un iversity, N ati nalK ey Lab f A gr m icr bi l gy, W uhan 430070, Ch ina) Ab stract: C pper-resistance related genes c pA and c pB w ere amplified by using the DNA as temp late ex- tracted fr m Xanth m nas citri subsp. citri. The target pr ducts w ere cl ned, and sequencing resu lts sh w s that sizes f c pA and c pB w ith 1 782 bp and 1 095 bp. The pr kary tic expressi n vect rs pET-c pA and pET-c pB f r these tw genesw ere c nstructed. B th c pA and c pB w ere effectively expressed as fusi n pr - teins in transf rm ed E scherich ia c li strain BL21(DE3) under the inducti n f IPTG. The expressed pr teins w ere ge-l purified and used t raise antisera in rabbits. The titers f antisera against rec mb inant pr teins f c - pA and c pB w ere 1B6 400 in ind irect-EL ISA. The expressed pr ducts w ere subjected t W e