宽叶泽苔草居群内遗传多样性的研究.pdfVIP

生物多样性 2005, 13 (5): 398–406 doi: 10.1360/biodiv.050133 Biodiversity Science http: // 宽叶泽苔草居群内遗传多样性研究 陈进明 王青锋* (武汉大学生命科学学院植物系统学与进化生物学研究室, 武汉 430072) 摘要: 采用RAPD分子标记对宽叶泽苔草(Caldesia grandis)湖南浪畔湖居群30个家系共180个子代样品进行了居群 内家系间以及家系内的遗传多样性分析。从100个随机引物中筛选出12个有效引物, 共产生112条带, 其中79条表 现出多态性, 占总条带数的70.5% 。宽叶泽苔草各个家系多态位点百分率(PPB)在7.1%–40.2%之间。各个家系基因 多样性范围在0.02–0.14之间(家系水平的基因多样性为0.10), Shannon指数的范围在0.04–0.21之间(家系水平的 Shannon指数为0.18) 。对宽叶泽苔草30个家系的AMOVA分析结果显示, 家系间的基因分化系数Gst = 0.231, 即总 的变异中有23.1% 的变异存在于家系间, 家系内的遗传变异占总遗传变异的76.9%, 家系间和家系内变异均极显著 (P0.001) 。采用UPGMA法对宽叶泽苔草30个家系180个子代进行聚类的结果表明: 同一家系的子代个体并不能完 全聚在一起。基于家系间的遗传分化系数Gst对宽叶泽苔草30个家系间的基因流进行估测, 结果显示: Nm = 0.83, 表 明宽叶泽苔草各个家系之间有较高的基因流。 关键词: 分子变异, Caldesia grandis, 濒危植物, 家系, RAPD Genetic diversity and structure in a natural Caldesia grandis population Jinming Chen, Qingfeng Wang* Laboratory of Plant Systematics and Evolutionary Biology, College of Life Sciences, Wuhan University, Wuhan 430072 Abstract: Genetic diversity in a natural Caldesia grandis population in Hunan Province was assessed using RAPD markers. A total of 180 individuals from 30 maternal plants were assayed. Of the 100 RAPD prim- ers screened, twelve produced highly reproducible bands. Using these primers, a total of 112 DNA frag- ments were generated with 79 (70.5%) being polymorphic. Analysis of molecular variance (AMOVA) show that a large proportion of genetic variation (76.9%) resided within families, while only a small pro- portion (23.1%) resided among families. Shannon index showed that total genetic div