热应激对小鼠睾丸Hsp70-2基因mRNA转录水平的影响.pdfVIP

安徽农业科学,Journal of Anhui Agri . Sci .2008 ,36(36) :15799 - 15801 责任编辑 李菲菲 责任校对 傅真治 热应激对小鼠睾丸 Hsp70- 2 基因 mRNA 转录水平的影响 姜忠玲 ( 青 岛农业大学动物科技学院, 山东青 岛266109) 摘要 用半定量 RT- PCR 方法检测不同温度、相 同时间(4 h) 热应激处理 小鼠睾丸组织中 Hsp70- 2 基因的mRNA 转录水平, 以研 究热应激 对 Hsp70- 2 基因mRNA 转录的影响。结果显示, 各热应激组小鼠睾丸 Hsp70- 2 mRNA 转录水平下降, 并且温度越 高, 其下降幅度越 大。与 对照组相比,33 ℃组小鼠 Hsp70- 2 mRNA 相对转录水平显著( P 0 .05) 下降, 而37 ℃组和42 ℃组下降极显著( P 0 .01) 。42 ℃组 Hsp70- 2 mRNA 相对转录水平仅为对照组的44 .7 % 。上述结果说明, 热应激使 Hsp70- 2 基 因mRNA 转录水平下降, 热应激对雄 性生殖机 能的影 响与 Hsp70-2 减少有关, 而 Hsp70-2 mRNA 转录水平可 以作为热应激对雄性生殖机 能损伤程度的判 断指标之一。 关键词 热应激; 小鼠; 睾丸;RT- PCR ; Hsp70-2 mRNA + 中图分类号 S865 . 1 3 文献标识码 A 文章编号 0517 - 6611(2008) 36 - 15799 - 03 Effects of Heat Stress on the Transcriptional Level of mRNA of Hsp70-2 Gene in Mice Testis JI ANG Zhong-ling ( College of Ani mal Science and Veterinary Medicine ,Qingdao Agricultural University ,Qingdao ,Shandong 266109) Abstract Semi- quantitative RT- PCR method was used to detect the transcriptional level of mRNA in Hsp70- 2 gene in testis tissue of mice which were treated at different temperature for the same ti me of heat stress .The effects of heat stress on mRNA transcription of Hsp70- 2 gene were studied .The results showed the transcriptional level of mRNA in Hsp70- 2 gene i n mice testis in each heat stress group decreased . And the higher the temperature was ,the greater decreasi ng extent of transcription level was .Compared with control group ,the relative transcriptional level of Hsp70- 2 mRNA in mice significantly decreased in 33 ℃group ( P 0 .05) and that extremely significantly decreased in the groups of 37 ℃and 42 ℃( P 0 .01) .The relative transcri ptional level of Hsp70-2 mRNA in 42 ℃group was 44 .7 % of that in the control group .The above results indicated that heat stress decreased the tr