微丝解聚剂细胞松弛素B以及微管阻断剂秋水仙素对藓羽藻细胞重建过程的影响.docVIP

 微丝解聚剂细胞松弛素 B 以及微管阻断剂 秋水仙素对藓羽藻细胞重建过程的影响# 毛玉峰，董文，刘玉娇，张真** 5 10 15 20 25 30 35 40 （中国海洋大学海洋生命学院 青岛 266003） 摘要：部分海洋多核绿藻原生质挤出加入到人工海水中细胞器即聚集，接着细胞组分重建， 细胞膜与细胞壁重新形成，这些重建的细胞在最佳条件下 40%能够发育成成熟藻体。细胞 骨架系统是细胞内有序性的维系者，也是细胞及细胞器运动的动力源，这些过程都涉及细胞 骨架的解聚与重聚合的动态过程。本论文利用微丝解聚剂细胞松弛素 B 以及微管阻断剂秋 水仙素对藓羽藻(Bryopsis hypnoides)细胞重建过程的影响进行了研究，结果显示不同浓度的 两种阻抑制均未对细胞重建的细胞器聚集过程产生明显影响，而高浓度的细胞松弛素 B （1mg/mL）对细胞器的聚集具有显著的促进作用，这说明细胞器聚集以及聚集体变圆的动 力不是来自与细胞骨架系统，而原始微丝体系的解聚是细胞器高效聚集的基本条件。然而， 细胞松弛素 B 以及秋水仙素都显著抑制细胞膜的形成以及细胞内组分有序性重建等后继发 育过程。 关键词：细胞重建；细胞骨架；细胞松弛素 B；秋水仙素；藓羽藻 中图分类号：Q26 Effects of Actin Cytoskeleton Depolymerize Reagent Cytochalasin B and Microtubule Disrupt Reagent Colchicine on Cell Reconstruction of Bryopsis Hypnoides MAO Yufeng, DONG Wen, LU Yujiao, ZHANG Zhen (College of Marine Life Sciences,Ocean University of China,Qingdao, 266003) Abstract: When some coenocytic algae were cut and the cytoplasmic contents squeezed out of the cell and into artificial sea water，the organelles begin to aggregate, and the contents of the cytoplasm become restructured, with a new cell membrane and wall forming for some of the cell. Under optimal conditions, 40% of these newly formed cells can develop into mature algal thalli. Cytoskeleton maintains and reorganizes the cellular architecture and mediates cell motility. Many of these processes require the dynamic behavior of the cytoskeleton which involves the polymerization and depolymerization. In the present study, the effects of actin cytoskeleton depolymerize reagent cytochalasin B and microtubule disrupt reagent colchicine on cell reconstruction of Bryopsis hypnoides have been studied. The results show that different concentrations of all those two regents do not have significant inhibitory effect on organelles aggregation, and interestingly, high concentration of cytochalasin B（1mg/mL）could promote this process, indicated that the motility of organelles during their aggregation were not drive by cytoskeleton system, and depolymerization of the original actin fil