深低温停循环对大鼠海马神经元线粒体超微结构的影响和定量分析.docVIP

 深低温停循环对大鼠海马神经元线粒体超 微结构的影响和定量分析# 5 10  摘要：目的 观察深低温停循环后大鼠海马神经元线粒体的超微结构改变，同时对超微结构 的变化进行定量分析。方法 取 SD 大鼠 40 只，随机分为 3 组：深低温停循环(DHCA)组、 常温停循环(NTCA)组和正常对照(NC)组(N=12)。采用体外插管法建立大鼠闭胸式体外循环 模型，DHCA 组体外循环建立后在深低温(20)条件下停循环 10 分钟；NTCA 组在正常温 度(36.8~37℃)条件下停循环 10 分钟；NC 组建立体外循环后不进行降温和停循环处理， 维持正常温度(36.8~37℃)体外循环 10 分钟。之后迅速断头取海马，制成透射电镜标本， 观察线粒体超微结构并进行体视学分析，结果采用 SPSS11.5 软件进行统计学分析。结果 线 粒体周长、直径、平均灰度及三维参数 Vv、Nv、V、δ在 NTCA 组分别与 NC 组和 DHCA 15 20 25 30 35 40 45 组比较有统计学差异(P＜0.05)。结论 DHCA 可以保持海马线粒体二维和三维参数的多个指 标相对稳定，减轻线粒体肿胀程度，促进能量代谢。 关键词：海马;深低温停循环;线粒体;超微结构;定量分析 中图分类号：R392.12 Quantative Analysis and Ultrastructural Observation of Mitochondria in Hippocampal Neurons of Rats under Whole Body Deep Hypothermic Circulatory Arrest GAO Yongjun, CAO Yi, CHEN Feixiong, FANG Shaolong, ZHAO Jinxi, LI Xuhui, XU Wei (Department of neurosurgry, the 2nd affiliated hospital of Kunming Medical University, Kunming 650101) Abstract: Objective To investigate the effects of deep hypothermic circulatory arrest at subcellular level by quantitative analysis and ultrastructural observation on hippocampal mitochondrion of rats.Methods Forty Sprague-Dawley (SD) rats were divided randomly into three groups: deep hypothermic circulatory arrest (DHCA) group, normal temperature circulatory arrest (NTCA) group and normal control (NC) group(n=12). The animal model of closed-chest cardiopulmonary bypass (CPB) was established in SD rats by means of external cannulation. In DHCA group,circulatory arrest of 10min were performed in the deep hypothermia (＜20℃); in NTCA group, the same operation were performed in normal temperature(36.8℃~37℃)and without circulatory arrest in normal temperature(36.8℃ ~37℃ )in NC group. And then the hippocampus were taken out of from the rats brain and were observed by electro microscope.There were some indexes were emphasized on the ultrastructure of the mitochondria of hippocampus, such as the circumference, the diameter, the mean gray scale, the volume density (Vv), the numerical density (N