蛋白疫苗免疫诱导Tfh细胞增强体液免疫水平的研究.docVIP

 蛋白疫苗免疫诱导 Tfh 细胞增强体液免疫 水平的研究# 张婧瑶，高文娟，黄波波，康友敏** 5 10 15 20 25 30 35 40 （中国农业大学生物学院，农业生物技术国家重点实验室，北京 100193） 摘要：目的： 研究蛋白疫苗免疫诱导产生滤泡辅助性 T 细胞（Tfh）增强 B 细胞功能，提高 疫苗的体液免疫水平。方法： 将他克莫司（FK506）作为佐剂与血吸虫重组蛋白（rSj32） 细 胞仪检测 Tfh 亚群的比例、生发中心（GC）的 B 细胞及 B 细胞表面分子的表达；通过胞内抗 体染色流式细胞仪方法检测 Tfh 细胞因子。结果： 免疫后的小鼠血清中的 IgG 抗体水平与 对照组相比显著增高，并且 GC 的 B 细胞显著增多；免疫后的小鼠脾脏中的 Tfh 细胞比例与 对照组相比显著增加，并且 Tfh 细胞所分泌的 IL-21 与对照组相比显著增加；免疫后的小鼠 脾脏中的 B 细胞表达 IL-21R 比例与对照组相比显著增高。结论： FK506 作为蛋白疫苗佐剂 能够诱导 Tfh 细胞的产生，Tfh 细胞可能通过产生 IL-21 与 B 细胞表面的 IL-21R 相互作用， 增强 B 细胞的功能，进而增强蛋白疫苗的体液免疫水平。 关键词：动物医学；蛋白疫苗；佐剂；Tfh 细胞；体液免疫 中图分类号：R392.1 The induction of Tfh cells by protein vaccine for enhancement of humoral immune responses ZHANG Jingyao, GAO Wenjuan, HUANG bobo, KANG Youmin (State Key Laboratory for Agro-Biotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193) Abstract: Objective: In this paper, we aimed to study the induction of follicular helper T (Tfh) cells by protein vaccine which can enhance the function of B cells and improve the humoral immunity. Methods: The BALB/C mice were immunized with Schistosoma Japonicum recombinant protein (rSj32) combined with Tacrolimus (FK506) twice. After the second immunization, the serum was collected for IgG level by ELISA. The splenocytes were prepared for Tfh cell subset, B cells in germinal center (GC) and the surface molecules on B cells by immunostaining with antibodies and analyzed by FACS. The expression of cytokines from Tfh cells were tested by intracellular staining with antibodies and analyzed by FACS. Results: The IgG level in immunized mice were significant increased and B cells in GC were increased compared with the control groups. The percentage of Tfh cells in spleens and also the IL-21 expression of Tfh cells were significant increased compared with the control groups. Furthermore, the IL-21R expressed on B cells was increased compared with the control groups. Conclusion: Protein vaccine with FK506 as adjuvant can induce Tfh cells. The