邻苯二甲酸单丁酯对支持细胞中GPR30基因表达的影响.docVIP

 Effect of Monobutyl phthalate on the expression of GPR30 gene in Sertoli cells# HU Yang1,2,3, LU Yuqiu1,2,3, LI Dongmei1,2,3, HAN Xiaodong1,2,3** 5 10 15 20 25 30 35 40 (1. Immunology and Reproduction Biology Laboratory, Medical School, Nanjing University, NanJing 210093; 2. Jiangsu Key Laboratory of Molecular Medicine, Nanjing University, NanJing 210093; 3. State Key Laboratory of Analytical Chemistry for Life Science, Nanjing University, NanJing 210093) Abstract: Recent studies indicated that the rapid and nongenomic action of xenoestrogen may be mediated through the membrane estrogen receptor-G protein-coupled receptor (GPR30). In this study we aimed to explore whether GPR30 is expressed in rat testicular Sertoli cells and the possible effect of monobutyl phthalate (MBP) on the expression of GPR30 in Sertoli cells. By using Rt-PCR, Western-Blot and immunocytochemistry, we assessed the expression of GPR30 in both gene and protein level. Then we examined the change of gene level of GPR30 in Sertoli cells exposed to MBP (0.01-1mM) for24h. The results indicated that GPR30 was expressed in rat testicular Sertoli cells and increased in gene level in Sertoli cells following administration with MBP even at a relative low concentration. We suppose that change of GPR30 expression may play an important role in effect of xenoestrogen MBP on Sertoli cells. Keywords: GPR30; monobutyl phthalate; Sertoli cells; nongenomic action 0 Introduction Endocrine disrupting chemicals (EDCs) are hormone-like agents that interfere with the synthesis, secretion, transport, binding, action, or elimination of natural hormones in the body that are responsible for development, behavior, fertility, and maintenances of homeostasis [1]. In particular, xenoestrogens are a type of EDCs that may imitate the nature hormone 17β-estradiol (E2) and have hormone disruptive effect on both wildlife and humans [2]. Xenoestrogens which are widely used in various industrial compounds, such as polychlorina