白藜芦醇对肥胖小鼠氧化还原状态和血压的影响.docVIP

 白藜芦醇对肥胖小鼠氧化还原状态和血压 的影响# 周青青，白静，施用晖，乐国伟** 5 10 15 20 25 30 35 （江南大学食品学院食品营养与功能因子研究所，无锡，214122） 摘要：目的 研究白藜芦醇(resveratrol,RSV)对高脂性肥胖小鼠抗氧化能力和血压调节的作用 及可能机制。方法 将 4 周龄雄性昆明种小鼠随机分成正常膳食组和高脂膳食 (high fat die,HFD)组。到第 12 周时从高脂膳食组中选出高体重小鼠构成肥胖(Obesity)模型，再分成 Obesity、Obesity+0.03%RSV、Obesity+0.06%RSV 三个膳食组，与正常组一起继续喂养 17 周。结果 与肥胖组相比，0.06%RSV 干预的小鼠体重显著降低，心组织抗氧化能力明显增 强，炎症因子 TNF-α 、IL-6 和 MCP-1 的含量显著降低(P﹤0.05)，血管收缩活性因子血管紧 张素和内皮素的含量显著降低(P﹤0.05)，显著降低平均动脉压(MBP)(P﹤0.05)至接近正常 组水平。其中氧自由基(ROS)、总抗氧化能力(T-AOC)、过氧化氢酶(CAT)、丙二醛(MDA)、 血管紧张素(AngⅡ)都与 MBP 存在相关关系。结论 添加适量白藜芦醇可以有效抵抗高脂 性肥胖小鼠的血压升高，其机制可能与抗氧化、抗炎症反应和调节血管活性物质含量有关。 关键词：白藜芦醇；肥胖；抗氧化；炎症；血压 中图分类号：R151.1 Effects of Resveratrol on Redox State and Blood Pressure Regulation in Obese Mice ZHOU Qingqing, ZHOU Qingqing, SHI Yong Hui, LE Guowei (The Research Center of Food Nutrition and Functional Factors,Food College,JiangNan University,WuXi,214122) Abstract: Purpose To study effects and mechanisms of resveratrol(RSV) in high-fatted mice. Methods 4-week-old mice were divided into normal diet group as control and high-fat diet(HFD) group at random. In the 12th week, high body weight mice were choosed from high-fat diet group and formed obesity model. They were divided into 3 diet groups such as Obesity, Obesity+0.03%RSV and Obesity+0.06%RSV, and were fed another 17 weeks together with control. Results: Compared with Obesity, 0.06%RSV treatment markedly depressed body weight, obviously depressed antioxidant obilities of cardiac tissue, and also significantly reduced(P﹤ 0.05)contents of inflammatory factors like TNF-α, IL-6, MCP-1 and vasoactive factors like Ang Ⅱ , ET in cardiac tissue. 0.06%RSV treatment significantly reduced(P﹤ 0.05)mean arterial pressure(MBP) to approach the lever of control group. RSV had doseeffect relationship.Therein, ROS, T-AOC, CAT, MDA, Angall had correlativity with MBP. Conglutions Adding proper dose RSV could effectively resist blood pressure rise in high-fatted mice, and the machanisms might involve with its antioxidanti