利用电形成法在超微电极上构建囊泡的研究.docVIP

 利用电形成法在超微电极上构建囊泡的 研究# 景婧1，展思辉2，张学军3，李轶1** 5 10 15 20 25 30 35 （1. 天津大学理学院，天津 300072； 2. 南开大学环境科学与工程学院，天津 300071； 3. 北京化工大学材料科学与工程学院，北京 100029） 摘要： 利用电形成法构建了 10-100μm 的巨型单层囊泡（GUVs），其与真核细胞大小相同 而且具有与细胞膜相似的封闭双层结构，被广泛用作细胞膜的模型和药物载体。本文突破传 统电极的限制，首次将碳纤维超微电极应用于电形成法制备囊泡的研究中。通过在碳纤维和 铂丝两平行电极施加电位峰峰值 Epp = 0.212-20V/mm，频率 f = 1-3000Hz 的交流电场，在 水溶液中调控囊泡的形成。并通过倒置光学显微镜实时原位考察了交流电位和频率对囊泡形 成的影响。研究发现在 1.5V 恒定电压下，当频率大于 1000Hz 时形成囊泡的数量逐渐减少， 囊泡的形成速率随着频率的增加而加快。保持 20Hz 频率，改变一系列电压，电压过高或过 低均不利于囊泡的形成，囊泡形成的最佳电位是 5V。 关键词：囊泡；超微电极；碳纤维；电形成法 中图分类号：O6 Construction of Giant Vesicles on the Ultramicroelectrode of Carbon Fibers Jing Jing1, Zhan Sihui2, Zhang Xuejun3, Li Yi1 (1. School of Science, Tianjin University, TianJin 300072; 2. The College of Environmental Science and Engineering, Nankai University, TianJin 300071; 3. College of Materials Science and Engineering, Beijing University of Chemical Technology, Beijing 100029) Abstract: Giant unilamellar vesicles（GUVs）of 10-100μm were prepared by electroformation method. Because GUVs are same as the size of eukaryotic cells and have the similar lilayer structure of the cell membrane, they can be widely used as the model of cell membrane and drug carrier. This paper breaks through the limit of the traditional electrode, and it first applies carbon fibers into the electroformation of vesicles. In this work, giant vesicles were obtained over a wide frequency range of 1-3000Hz and peak-to-peak value from 0.212V/mm to 20V/mm between two parallel conducting electrodes of carbon fibers and a platinum wire under alternative current (AC) electric field. The influence of AC voltage and frequency on the structural evolution of giant vesicles was in situ observed by inverted optical microscope. With the increase of the frequency, the number of vesicles gradually decreases and the rate of the vesicle formation increases when the frequency is greater than 1000Hz under the constant voltage of 1.5V. What’s more, it is found that the best potential parameter of t