顶空气相色谱法测定川芎栽培地土壤中的挥发性有机物.docVIP

 顶空气相色谱法测定川芎栽培地土壤中的 挥发性有机物*# * 5 10 15 20 25 30 35  摘要：目的 通过比较川芎药材栽培地与苓种繁殖地不同环境土壤有机化学成分的变化，研 究川芎栽培地土壤有机物种类及土壤安全性，初步评价川芎生长地土壤的质量。方法 采用 常规方法取川芎 10、20、30cm 耕层土和根际土土壤，土样及时送回实验室-4保存；GC/MS 色谱条件：HP-INNOWAX(50m×0.25mm×0.25um)毛细管色谱柱，升温程序：50（3min） 以 14/min 升至 150，再以 10/min 升至 260，恒温 5min；载气为氦气；流速 1.4ml/min； 质谱条件：EI,70ev，扫描范围：20～450amu；处理方式：用 SPME(非极性)顶空进样，顶空 温度：120，时间：40min；富集时间：20min；脱附时间：5min。结果 从 2011 年三个产 地 9 份土样中检测确认 70 个成分；从部分土样中检出了甲苯、1-氯-甲苯、1-甲基-4-甲乙基 -苯、1,2-二氯苯等 12 种苯系物，相对含量较高的有 1-甲基-4-（1-甲乙烯基）-苯（benzene， 1-methyl-4-（1-methyletheny），1-甲基-3-（1-甲乙基）-苯（benzene，1-methyl-3-（1-methylethy）， 甲苯（benzene，methyl－）等；不同土层深度有机物种类差别较大，根际土与非根际土有机 物种类差别较大，川芎种苓繁殖地土壤有毒有机物种类较药材栽培地少。结论 川芎种苓与 药材生长地土壤的有机成分多样化明显，相对含量变化较大；种苓繁殖地土壤受有毒有机物 污染较少，药材栽培地部分土壤被苯系物污染，土壤质量和安全性受到影响，亟需深入评价 研究。 关键词：川芎；土壤成分；苯系物；顶空气相色谱 中图分类号：R282.2 Determination of Soil Volatile Compounds in Habitat of Ligusticum chuanxiong Hort. by Headspace Gas Chromatography WANG Guangzhi1, YAN Zhuyun2 (1. Pharmacy School of Chengdu University of TCM,Chengdu 611137; 2. Pharmacy School of Chengdu University of TCM, Chengdu 611137) Abstract: Objective：To preliminary evaluate the soil quality and soil degradation status in habitat of Ligusticum Chuanxiong by determination of chemical composition and secondary pollutants of the soil. Methods: 10,20 and 30 cm topsoil and rhizosphere soil samples were collected and preserved in time back to the lab -4 ℃; The GC-MS chromatography warming program was : 50℃（3min）and then warming to 150, then warming to 260℃ at the rate of 10℃/min and keeping constant temp. for 5min, the gas flow rate was 1.4ml/min; EI wsa 70ev and the scanning range was 20～450amu. All samples were headspaced injected with SPME, the injected temp. was -1-  120.keeping time: 40min, accumulation time: 20min and the desorption time: 5min. Results: 70 40 45 components were confirmed from 9 sapmles collected in 3 different habitats, among which 12 toxic compounds such as benzene,1-methyl-4- （ 1-methyletheny ） , methyl-benzene