OXA介导的CRAB.docVIP

ORIGINAL ARTICLE Year : 2011 | Volume : 29 | Issue : 3 | Page : 269-274 OXA beta-lactamase-mediated carbapenem resistance in Acinetobacter baumannii SM Amudhan1, U Sekar1, K Arunagiri2, B Sekar3 1 Department of Microbiology, Sri Ramachandra Medical College and Research Institute, Sri Ramachandra University, Porur, Chennai, India 2 Department of Molecular Biology, Department of Microbiology, Central Leprosy Teaching and Research Institute, Chengalpattu, India 3 Pasteur Institute of India, Coonoor, Nilgiris, Tamil Nadu, India Date of Submission 21-Apr-2011 Date of Acceptance 01-Jul-2011 Date of Web Publication 17-Aug-2011 Correspondence Address: U Sekar Department of Microbiology, Sri Ramachandra Medical College and Research Institute, Sri Ramachandra University, Porur, Chennai India DOI: 10.4103/0255-0857.83911 PMID: ~ Abstract Objectives: Acinetobacter baumannii is a significant pathogen in health care settings. In recent years, an increase in carbapenem resistance among A. baumannii due to Ambler class B metallo-beta-lactamases or class D OXA carbapenamases has been reported. In this study we detected the presence of OXA carbapenamases and coproduction of metallo-beta-lactamases (blaVIM and blaIMP ) by phenotypic and genotypic methods in carbapenem resistant clinical isolates of Acinetobacter baumannii. Materials and Methods: A total of 116 consecutive, non-duplicate carbapenem resistant A. baumannii isolated from various clinical specimens were included in the study. The modified Hodge test and inhibitor potentiated disk diffusion tests were done for the screening of carbapenamase and metallo-beta-lactamase production, respectively. Polymerase chain reaction (PCR) was performed for the detection of OXA (blaOXA 23 like, blaOXA 24 like, blaOXA-51 like and blaOXA-58 like genes) and metallo-beta-lactamases (blaVIM and blaIMP ) genes. Gene sequencing was performed for representative isolates. Results: Among 116 A. baumannii, OXA genes were dete