毕业设计：酸枣酚类化合物的高效液相色谱测定及其抗氧化作用的研究（终稿）.doc

PAGE  PAGE 22 酸枣酚类化合物的高效液相色谱测定及其抗氧化作用的研究 （西北大学 化工学院食品科学与工程系 西安 710069） 摘要：本文以3种不同溶剂酸枣提取物为研究对象，建立了一种测定酸枣酚类化合物的HPLC-ECD分析方法以及在测定其提取物中总酚酸和总黄酮含量的基础上，利用体外法测定各提取物的还原能力、对Fe2+的络合能力、对DPPH自由基的清除作用及对羟基自由基的清除作用。实验结果表明，酸枣中含有没食子酸、原儿茶酸、咖啡酸、p-香豆酸、阿魏酸、芦丁、槲皮素、杨梅酮、高良姜素、山奈酚等十种酚酸。3种不同溶剂酸枣提取物均有显著的抗氧化性，呈剂量效应关系，酚酸含量、还原能力、抗氧化能力之间呈正相关。 关键词：酸枣；酚类化合物；高效液相色谱；抗氧化 Jujube phenolic compounds by HPLC and its antioxidant research (Northwest University, College of Chemical Engineering, Food Science Technology, Xi’an 710069) Abstract: In this paper, three kinds of extracts in different solvents axillaris study, the establishment of a determination of phenolic compounds in jujube HPLC-ECD method and measured the total phenolic extract and total flavonoids based on the in vitro method?The ability to extract the reduction of Fe2+complexation ability of the DPPH free radical scavenging and scavenging of hydroxyl radicals.?Experimental results show that the jujube contains gallic acid, protocatechuic acid, caffeic acid, p-coumaric acid, ferulic acid, rutin, quercetin, myricetin, galangin, kaempferol and ten other acids.?3 different solvents jujube extract showed a significant antioxidant activity in a dose-response relationship, phenolic content, reducing power, antioxidant capacity were positively correlated. Key words: Jujube; phenolic compounds; HPLC; antioxidant 目 录 摘要 Abstract  TOC \o 1-3 \h \z \u  HYPERLINK \l _Toc263334969 第一章 绪论  PAGEREF _Toc263334969 \h 1  HYPERLINK \l _Toc263334970 1.1 酚酸类化合物  PAGEREF _Toc263334970 \h 1  HYPERLINK \l _Toc263334971 1.2 黄酮类化合物  PAGEREF _Toc263334971 \h 1  HYPERLINK \l _Toc263334972 1.3 高效液相色谱检测器  PAGEREF _Toc263334972 \h 2  HYPERLINK \l _Toc263334973 1.3.1 电化学检测器  PAGEREF _Toc263334973 \h 2  HYPERLINK \l _Toc263334974 1.4 高效液相色谱法在酚酸和黄酮化合物中的应用  PAGEREF _Toc263334974 \h 2  HYPERLINK \l _Toc263334975 1.4.1 高效液相色谱法在酚酸和黄酮化合物检测中的应用  PAGEREF _Toc263334975 \h 3  HYPERLINK \l _Toc263334976 1.4.2 高效液相色谱法在酚酸