辽宁新品系绒山羊ZFXZFY基因片段的进化研.pdfVIP

, 2006, 37( 6) , 530~ 536 A cta Veterinaria et Zootechnica S inica ZFX ZF Y * , , , , ( , 116029) : ( M ouflo n) ( H uman) ZF X ZF Y , DN A , PCR ZFX ZF Y 11 , N CBI BL AST , Clus alX. ex eM eg aBioEdi . ex eD NA Club ( N ewbr eeding cashmere g oa ) ( T ibe an sheep) ( M ouflo n) ( Ho rse) ( Co yo e) ( P ig) ( Whale) ( Human) ( Cow ) ZFX ZF Y NJ : ZFX ZF Y 92% , 95% , , A vaII ZF X ZF Y PCR , A vaII ZFX , : ; ZF X ; ZF Y; ; ; : S826. 2 : A : 0 3666964( 2006) Cloning andEvolutionAnalysis of ZFX, ZF Y Partial Gene inNewbreeding Cash ereGoat andIdentification of Its Sex * JIN M ei , FU Z ho ngyang, L U A N Y uanyuan , GA O Wenbo, WA N G Wei ( Colleg e of L if e Science, L iaoning N omal Univ ersity , D al ian 116029, China Abstract: A cco rding o he M ouflo n and he hum an sex dif feren ia ion rela ed ZFX and ZF Y genes, a pair of primers w ere designed. T he f ragm en s w ere am plif ied fro m he genomic DNA of male or f em ale N ewbreeding cashmere g oa . Subsequen ly he por ion am plif ied f ragmen s of ex o n 11 w ere cloned and sequenced . T he comparison of hese sequences w i h hose of o her animals ( T ibe an sheep, M ouf lon, H orse, Co yo e, Pig, Whale, H uman and Cow ) w as perf orm ed using he bioso f w are such as, Clus alX . exe, M eg a hrough blas n o f N CBI. U sing m any w ays o build mo l ecule sys em rees , f or example, he NJ m e hod can show ha he newbreeding cashmere goa is he closes o M ouf lon, w hile i is he f ur hes o o her species . T he resul s of co mparison indi ca ed ha he homo log ies o f nucleo ide sequence is 92% , he